电离辐射通过转化生长因子-β-介导的内膜-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

内容可 ：聚焦远红外线是否可通过转化生长因子-β（TGF-β）-依赖性的视网膜-游离转换 (EMT)来倡导细胞内的蹂躏移入。用到总量2Gy(60)Coγ线太阳光源自人类器官的6种细胞内，据信与EMT具体的变化，这最主要分别借助于显微镜技术，蛋白质区别于方法律条文，免疫荧光技术，水痘试验中和Transwell鲍洛通试验中来观察并检验细胞内组织构造，EMT上标，蹂躏移入能力也等。改用酶联免疫吸附法律条文检验这些细胞内中TGF-β蛋白水平，借助于值得注意消除剂SB431542来风险评估TGF-β信号通路在远红外线EMT中的作用。经过总量为2Gy太阳光的细胞内中不存在间叶细胞内的隐含，与假太阳光组相比其视网膜上标减少，间叶细胞内上标增加，同时其蹂躏转移能力也加强，TGF-β蛋白水平也增加。进一步断定由A549远红外线诱发的EMT可通过对TGF-β信号消除遭遇逆转。这些最近TGF-β依赖性的EMT在远红外线诱发加强细胞内蹂躏转移能力也中起着关键作用。

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